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Overview
Product name
Anti-Ube2N / Ubc13 antibody
See all Ube2N / Ubc13 products (8) ...
Description
Rabbit polyclonal to Ube2N / Ubc13
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to 15 amino acids near the C terminus of Human Ube2N.
Positive control
Human small intestine or HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: PBS.
Concentration
Concentration information loading...
Purification notes
This antibody was purified by Ion exchange chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Ubiquitin & Ubiquitin Like Modifiers >> E2 Ubiquitin Conjugating Enzymes
Immunology >> Innate Immunity >> TLR Signaling
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Ubiquitin E2 Enzymes
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1.0 µg/ml.Predicted molecular weight: 18 kDa.
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: Use a concentration of 2 µg/ml
Target
Function
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational
modifications
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionP61088
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
Database links
- Entrez Gene: 7334 Human
- Entrez Gene: 93765 Mouse
- Entrez Gene: 116725 Rat
- Omim: 603679 Human
- SwissProt: P61088 Human
- SwissProt: P61089 Mouse
- SwissProt: Q9EQX9 Rat
- Unigene: 524630 Human
see all
Anti-Ube2N / Ubc13 antibody images:
Western blot - Ube2N antibody (ab25885)
All lanes : Anti-Ube2N / Ubc13 antibody (ab25885) (analysis
in human
small intestine cell lysates 15ug/lane
)
Lane 1 : 0.5 ug/ml of antibody.
Lane 2 : 1 ug/ml of antibody.
Lane 3 : 2 ug/ml of antibody.
Predicted band size : 18 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ube2N antibody (ab25885)
ab25885 at 2µg/ml staining UBe2N in mouse thymus tissue by IHC
Immunocytochemistry/ Immunofluorescence-Ube2N / Ubc13 antibody(ab25885)
ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Ube2N / Ubc13 antibody (ab25885)
ab25885 has not yet been referenced specifically in any publications.
Publishing research using ab25885? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"