Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)

Overview

  • Product nameAnti-Ube2N / Ubc13 antibody [EPR5162]
    See all Ube2N / Ubc13 primary antibodies
  • Description
    Rabbit monoclonal [EPR5162] to Ube2N / Ubc13
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Ube2N/ Ubc13

  • Positive control
    • Human testis tissue. Jurkat, Daudi and Human heart lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionThe UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
    • Post-translational
      modifications
      Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
    • Cellular localizationNucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Bendless like ubiquitin conjugating enzyme antibody
      • Bendless-like ubiquitin-conjugating enzyme antibody
      • BLU antibody
      • EC 6.3.2.19 antibody
      • Epididymis secretory protein Li 71 antibody
      • HEL-S-71 antibody
      • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
      • MGC131857 antibody
      • MGC8489 antibody
      • UBC 13 antibody
      • Ubc13 antibody
      • UbCH ben antibody
      • UbcH-ben antibody
      • UbcH13 antibody
      • UBCHBEN antibody
      • Ube 2N antibody
      • Ube2n antibody
      • UBE2N_HUMAN antibody
      • Ubiquitin carrier protein N antibody
      • Ubiquitin conjugating enzyme E2 N antibody
      • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
      • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2N antibody
      • Ubiquitin protein ligase N antibody
      • Ubiquitin-conjugating enzyme E2 N antibody
      • Ubiquitin-protein ligase N antibody
      • Yeast UBC13 homolog antibody
      see all

    Anti-Ube2N / Ubc13 antibody [EPR5162] images

    • All lanes : Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Human heart lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 17 kDa
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109286 at 1/100 dilution.

    References for Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)

    ab109286 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"