Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)


  • Product nameAnti-Ube2N / Ubc13 antibody [EPR5163]
    See all Ube2N / Ubc13 primary antibodies
  • Description
    Rabbit monoclonal [EPR5163] to Ube2N / Ubc13
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Ube2N/ Ubc13.

  • Positive control
    • WB: Jurkat, HeLa, Raji and 293T cell lysates Flow Cyt: Jurkat cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab109294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionThe UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
    • Post-translational
      Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
    • Cellular localizationNucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Bendless like ubiquitin conjugating enzyme antibody
      • Bendless-like ubiquitin-conjugating enzyme antibody
      • BLU antibody
      • EC antibody
      • Epididymis secretory protein Li 71 antibody
      • HEL-S-71 antibody
      • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
      • MGC131857 antibody
      • MGC8489 antibody
      • UBC 13 antibody
      • Ubc13 antibody
      • UbCH ben antibody
      • UbcH-ben antibody
      • UbcH13 antibody
      • UBCHBEN antibody
      • Ube 2N antibody
      • Ube2n antibody
      • UBE2N_HUMAN antibody
      • Ubiquitin carrier protein N antibody
      • Ubiquitin conjugating enzyme E2 N antibody
      • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
      • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2N antibody
      • Ubiquitin protein ligase N antibody
      • Ubiquitin-conjugating enzyme E2 N antibody
      • Ubiquitin-protein ligase N antibody
      • Yeast UBC13 homolog antibody
      see all

    Anti-Ube2N / Ubc13 antibody [EPR5163] images

    • All lanes : Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • Flow cytometric analysis of Ube2N / Ubc13 in permeabilized Jurkat cells, using ab109294 at a dilution of 1/250 (red) or a rabbit IgG (negative) (green).

    References for Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)

    ab109294 has not yet been referenced specifically in any publications.

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