Recombinant
RabMAb

Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294)

Overview

  • Product name
    Anti-Ube2N / Ubc13 antibody [EPR5163]
    See all Ube2N / Ubc13 primary antibodies
  • Description
    Rabbit monoclonal [EPR5163] to Ube2N / Ubc13
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Ube2N/ Ubc13.

  • Positive control
    • WB: Jurkat, HeLa, Raji and 293T cell lysates Flow Cyt: Jurkat cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the ubiquitin-conjugating enzyme family.
    • Post-translational
      modifications
      Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
    • Cellular localization
      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Bendless like ubiquitin conjugating enzyme antibody
      • Bendless-like ubiquitin-conjugating enzyme antibody
      • BLU antibody
      • EC 6.3.2.19 antibody
      • Epididymis secretory protein Li 71 antibody
      • HEL-S-71 antibody
      • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
      • MGC131857 antibody
      • MGC8489 antibody
      • UBC 13 antibody
      • Ubc13 antibody
      • UbCH ben antibody
      • UbcH-ben antibody
      • UbcH13 antibody
      • UBCHBEN antibody
      • Ube 2N antibody
      • Ube2n antibody
      • UBE2N_HUMAN antibody
      • Ubiquitin carrier protein N antibody
      • Ubiquitin conjugating enzyme E2 N antibody
      • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
      • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2N antibody
      • Ubiquitin protein ligase N antibody
      • Ubiquitin-conjugating enzyme E2 N antibody
      • Ubiquitin-protein ligase N antibody
      • Yeast UBC13 homolog antibody
      see all

    Images

    • All lanes : Anti-Ube2N / Ubc13 antibody [EPR5163] (ab109294) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • Flow cytometric analysis of Ube2N / Ubc13 in permeabilized Jurkat cells, using ab109294 at a dilution of 1/250 (red) or a rabbit IgG (negative) (green).

    References

    ab109294 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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