Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab76653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the covalent attachment of ubiquitin to other proteins.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt
  • Database links
  • Alternative names
    • RP4-636O23.1 antibody
    • UBE2U antibody
    • UBE2U_HUMAN antibody
    • Ubiquitin carrier protein U antibody
    • Ubiquitin conjugating enzyme E2U (putative) antibody
    • Ubiquitin-conjugating enzyme E2 U antibody
    • Ubiquitin-protein ligase U antibody
    see all

Anti-UBE2U antibody images

  • Anti-UBE2U antibody (ab76653) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab76653 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76653, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-UBE2U antibody (ab76653)

ab76653 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"