Overview

  • Product nameAnti-UBE2V1 antibody
    See all UBE2V1 primary antibodies
  • Description
    Rabbit polyclonal to UBE2V1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human UBE2V1 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q13404

  • Positive control
    • This antibody gave a positive signal in Human Pancreas tissue lysate as well as the following whole cell lysates: PANC1; HeLa; U20S; HEK293; HeLa Nuclear.

Properties

Applications

Our Abpromise guarantee covers the use of ab101476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Target

  • FunctionHas no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
  • Tissue specificityHighly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow and pancreas. Detected at low levels in heart, breast, placenta, brain, liver, kidney, stomach and lung.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationNucleus. Excluded from the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIR1 antibody
    • CROC-1 antibody
    • CROC1 antibody
    • CROC1A antibody
    • TRAF6-regulated IKK activator 1 beta Uev1A antibody
    • UB2V1_HUMAN antibody
    • UBE2V 1 antibody
    • UBE2V antibody
    • UBE2V1 antibody
    • Ubiquitin conjugating enzyme E2 variant 1 antibody
    • Ubiquitin-conjugating enzyme E2 variant 1 antibody
    • UEV-1 antibody
    • UEV1 antibody
    see all

Anti-UBE2V1 antibody images

  • All lanes : Anti-UBE2V1 antibody (ab101476) at 1 µg/ml

    Lane 1 : Pancreas (Human) Tissue Lysate - adult normal tissue
    Lane 2 : PANC-1 (Human Pancreatic Carcinoma) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 70 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab101476 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References for Anti-UBE2V1 antibody (ab101476)

ab101476 has not yet been referenced specifically in any publications.

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