Overview

  • Product nameAnti-UBE2V1 antibody
    See all UBE2V1 primary antibodies
  • Description
    Mouse polyclonal to UBE2V1
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human UBE2V1 (AAH00468).

  • Positive control
    • Human pancreas tissue lysate, UBE2V1 transfected 293T cell lysate and HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionHas no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
  • Tissue specificityHighly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow and pancreas. Detected at low levels in heart, breast, placenta, brain, liver, kidney, stomach and lung.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
  • Cellular localizationNucleus. Excluded from the nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIR1 antibody
    • CROC-1 antibody
    • CROC1 antibody
    • CROC1A antibody
    • TRAF6-regulated IKK activator 1 beta Uev1A antibody
    • UB2V1_HUMAN antibody
    • UBE2V 1 antibody
    • UBE2V antibody
    • UBE2V1 antibody
    • Ubiquitin conjugating enzyme E2 variant 1 antibody
    • Ubiquitin-conjugating enzyme E2 variant 1 antibody
    • UEV-1 antibody
    • UEV1 antibody
    see all

Anti-UBE2V1 antibody images

  • Immunohistochemistical detection of Anti-UBE2V1 antibody (ab88679; 1/50 for 1h @ 21°C) on human kidney formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 0.25% Casein in PBS for 10 mins at 21°C. Secondary antibody HRP conjugated Dako REAL EnVision Goat anti Rabbit/Mouse (undiluted).

    See Abreview

  • Anti-UBE2V1 antibody (ab88679) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Predicted band size : 16 kDa
    Observed band size : 16 kDa
    Additional bands at : 32 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-UBE2V1 antibody (ab88679) at 1 µg/ml

    Lane 1 : UBE2V1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 16 kDa
    Observed band size : 16 kDa
  • ab88679 at 10µg/ml staining UBE2V1 in HeLa cells by Immunofluorescence.

References for Anti-UBE2V1 antibody (ab88679)

ab88679 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Casein in PBS as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6
Sample Human Tissue sections (Kidney)
Specification Kidney
Permeabilization Yes - 0.25% Triton X-100
Fixative Formaldehyde
Username

Dr. Francesca Conserva

Verified customer

Submitted May 13 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"