The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Use a concentration of 10 µg/ml.
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionHas no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
Tissue specificityHighly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow and pancreas. Detected at low levels in heart, breast, placenta, brain, liver, kidney, stomach and lung.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
Cellular localizationNucleus. Excluded from the nucleolus.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2V1 antibody (ab88679)This image is courtesy of an abreview submitted by Francesca Conserva, Università degli Studi - Bari
Immunohistochemistical detection of Anti-UBE2V1 antibody (ab88679; 1/50 for 1h @ 21°C) on human kidney formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 0.25% Casein in PBS for 10 mins at 21°C. Secondary antibody HRP conjugated Dako REAL EnVision Goat anti Rabbit/Mouse (undiluted).