The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at a concentration of 1 - 4 µg/mg of cell lysate.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
PathwayProtein modification; protein ubiquitination.
Human papilloma virus E6 associated protein Angelman syndrome antibody
Human papilloma virus E6 associated protein antibody
Human papillomavirus E6-associated protein antibody
NY REN 54 antigen antibody
Oncogenic protein associated protein E6 AP antibody
Oncogenic protein associated protein E6AP antibody
Oncogenic protein-associated protein E6-AP antibody
Renal carcinoma antigen NY REN 54 antibody
Renal carcinoma antigen NY-REN-54 antibody
UBE 3A antibody
UBE3A protein antibody
Ubiquitin protein ligase E3A antibody
Ubiquitin-protein ligase E3A antibody
Anti-UBE3A antibody images
Immunoprecipitation - UBE3A antibody (ab10487)
ab10487 immunoprecipitation of UBE3A (E6AP).Samples: Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Antibody: ab10487 used at 20 µg/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is UBE3A (E6AP).
References for Anti-UBE3A antibody (ab10487)
has not yet been referenced specifically in any publications.
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