Overview

  • Product nameAnti-UBE3A antibody
    See all UBE3A primary antibodies
  • Description
    Rabbit polyclonal to UBE3A
  • Tested applicationsSuitable for: IP, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein (proprietary-tag) corresponding to Human UBE3A.

  • Positive control
    • WB: HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab3519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate. IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.

IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseAngelman syndrome
  • Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
  • Post-translational
    modifications
    Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANCR antibody
    • Angelman syndrome antibody
    • AS antibody
    • CTCL tumor antigen se37 2 antibody
    • CTCL tumor antigen se37-2 antibody
    • E6 AP antibody
    • E6AP antibody
    • E6AP ubiquitin protein ligase antibody
    • E6AP ubiquitin-protein ligase antibody
    • EPVE6AP antibody
    • FLJ26981 antibody
    • HPVE6A antibody
    • Human papilloma virus E6 associated protein Angelman syndrome antibody
    • Human papilloma virus E6 associated protein antibody
    • Human papillomavirus E6-associated protein antibody
    • NY REN 54 antigen antibody
    • Oncogenic protein associated protein E6 AP antibody
    • Oncogenic protein associated protein E6AP antibody
    • Oncogenic protein-associated protein E6-AP antibody
    • Renal carcinoma antigen NY REN 54 antibody
    • Renal carcinoma antigen NY-REN-54 antibody
    • UBE 3A antibody
    • Ube3a antibody
    • UBE3A protein antibody
    • UBE3A_HUMAN antibody
    • Ubiquitin protein ligase E3A antibody
    • Ubiquitin-protein ligase E3A antibody
    see all

Anti-UBE3A antibody images



  • Predicted band size : 100 kDa

    Western blot detection of UBE3A in HeLa cell extract using ab3519.

References for Anti-UBE3A antibody (ab3519)

ab3519 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. I have checked with the originator of this antibody and it does seem that the protein that was used was most likely degraded (when E6-AP degrades a 50 kDa band seen in Western blots). However, we really don't know why the othe...

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Thank you for the details that you have provided. Please note that E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots. This may explain why your...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"