• Product nameAnti-UBE3A antibody
    See all UBE3A primary antibodies
  • Description
    Rabbit polyclonal to UBE3A
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human UBE3A.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: T24/83; HeLa; HepG2; HEK293.



Our Abpromise guarantee covers the use of ab94493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).


  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseAngelman syndrome
  • Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
  • Post-translational
    Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANCR antibody
    • Angelman syndrome antibody
    • AS antibody
    • CTCL tumor antigen se37 2 antibody
    • CTCL tumor antigen se37-2 antibody
    • E6 AP antibody
    • E6AP antibody
    • E6AP ubiquitin protein ligase antibody
    • E6AP ubiquitin-protein ligase antibody
    • EPVE6AP antibody
    • FLJ26981 antibody
    • HPVE6A antibody
    • Human papilloma virus E6 associated protein Angelman syndrome antibody
    • Human papilloma virus E6 associated protein antibody
    • Human papillomavirus E6-associated protein antibody
    • NY REN 54 antigen antibody
    • Oncogenic protein associated protein E6 AP antibody
    • Oncogenic protein associated protein E6AP antibody
    • Oncogenic protein-associated protein E6-AP antibody
    • Renal carcinoma antigen NY REN 54 antibody
    • Renal carcinoma antigen NY-REN-54 antibody
    • UBE 3A antibody
    • Ube3a antibody
    • UBE3A protein antibody
    • UBE3A_HUMAN antibody
    • Ubiquitin protein ligase E3A antibody
    • Ubiquitin-protein ligase E3A antibody
    see all

Anti-UBE3A antibody images

  • All lanes : Anti-UBE3A antibody (ab94493) at 1 µg/ml

    Lane 1 : T24/83 (Human bladder carcinoma) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 101 kDa
    Observed band size : 101 kDa

    Exposure time : 8 minutes

References for Anti-UBE3A antibody (ab94493)

ab94493 has not yet been referenced specifically in any publications.

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