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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab74653 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 123 kDa.
Relevance UBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
Anti-UBE3B antibody images
Western blot - UBE3B antibody (ab74653)
All lanes : Anti-UBE3B antibody (ab74653) at 1/500 dilution Lane 1 : Jurkat cell extracts Lane 2 : Jurkat cell extracts with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 123 kDa Observed band size : 123 kDa Additional bands at : 30 kDa,48 kDa. We are unsure as to the identity of these extra bands.
References for Anti-UBE3B antibody (ab74653)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"