Overview

  • Product name
    Anti-UBE3B antibody
  • Description
    Rabbit polyclonal to UBE3B
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 720 - 769 (VDEAGIDQDG VFKEFLEEII KRVFDPALNL FKTTSGDERL YPSPTSYIHE) of Human UBE3B (NP_569733)

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 123 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    UBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
  • Database links
  • Alternative names
    • AI449831 antibody
    • AU020130 antibody
    • FLJ45294 antibody
    • MGC131858 antibody
    • MGC78388 antibody
    • Ubiquitin protein ligase E3B antibody
    see all

Images

  • Anti-UBE3B antibody (ab83834) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 123 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

References

ab83834 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83834.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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