Overview

  • Product name
  • Description
    Rabbit polyclonal to UBE3C
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human UBE3C aa 549-578 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q15386

  • Positive control
    • HeLa cell line lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab180113 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 124 kDa.

Target

  • Function
    E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
  • Tissue specificity
    Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 IQ domain.
  • Domain
    The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HectH2 antibody
    • KIAA0010 antibody
    • KIAA10 antibody
    • Ube3c antibody
    • UBE3C_HUMAN antibody
    • Ubiquitin protein isopeptide ligase (E3) antibody
    • Ubiquitin protein ligase E3C antibody
    • Ubiquitin-protein ligase E3C antibody
    see all

Images

  • Anti-UBE3C antibody (ab180113) at 1/1000 dilution + HeLa cell line lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 124 kDa

References

ab180113 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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