Overview

  • Product name
  • Description
    Mouse polyclonal to UBE3C
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length UBE3C protein (Human)

  • Positive control
    • UBE3C transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 124 kDa).

Target

  • Function
    E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
  • Tissue specificity
    Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 IQ domain.
  • Domain
    The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HectH2 antibody
    • KIAA0010 antibody
    • KIAA10 antibody
    • Ube3c antibody
    • UBE3C_HUMAN antibody
    • Ubiquitin protein isopeptide ligase (E3) antibody
    • Ubiquitin protein ligase E3C antibody
    • Ubiquitin-protein ligase E3C antibody
    see all

Images

  • All lanes : Anti-UBE3C antibody (ab68225) at 1/500 dilution

    Lane 1 : UBE3C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 124 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References

ab68225 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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