Overview

  • Product nameAnti-UBE3C antibody
    See all UBE3C primary antibodies
  • Description
    Mouse polyclonal to UBE3C
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length UBE3C protein (Human)

  • Positive control
    • UBE3C transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab68225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 124 kDa).

Target

  • FunctionE3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
  • Tissue specificityHighly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 IQ domain.
  • DomainThe C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HectH2 antibody
    • KIAA0010 antibody
    • KIAA10 antibody
    • Ube3c antibody
    • UBE3C_HUMAN antibody
    • Ubiquitin protein isopeptide ligase (E3) antibody
    • Ubiquitin protein ligase E3C antibody
    • Ubiquitin-protein ligase E3C antibody
    see all

Anti-UBE3C antibody images

  • All lanes : Anti-UBE3C antibody (ab68225) at 1/500 dilution

    Lane 1 : UBE3C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 124 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

References for Anti-UBE3C antibody (ab68225)

ab68225 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab68225.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"