Overview

  • Product nameAnti-UBE4B antibody
    See all UBE4B primary antibodies
  • Description
    Mouse monoclonal to UBE4B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: FKLLAEKVEE IVAKNARAEI DYSDAPDEFR DPLMDTLMTD PVRLPSGTIM DRSIILRHLL NSPTDPFNRQ TLTESMLEPV PELKEQIQAW MREKQNSDH, corresponding to amino acids 1075-1174 of Human UBE4B

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab56691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionBinds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
    • Tissue specificityHighest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
    • Sequence similaritiesBelongs to the ubiquitin conjugation factor E4 family.
      Contains 1 U-box domain.
    • Post-translational
      modifications
      Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930551I19Rik antibody
      • 4933406G05Rik antibody
      • AU014668 antibody
      • D4Bwg0973e antibody
      • E4 antibody
      • HDNB1 antibody
      • Homozygously deleted in neuroblastoma 1 antibody
      • KIAA0684 antibody
      • mKIAA0684 antibody
      • RP23 173D8.1 antibody
      • Ube4b antibody
      • UBE4B_HUMAN antibody
      • Ubiquitin conjugation factor E4 B antibody
      • Ubiquitin fusion degradation protein 2 antibody
      • UBOX3 antibody
      • UFD2 antibody
      • UFD2a antibody
      • Ufd2p antibody
      see all

    Anti-UBE4B antibody images



    • Predicted band size : 146 kDa

    References for Anti-UBE4B antibody (ab56691)

    ab56691 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (U2OS)
    Specification U2OS
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Username

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    Verified customer

    Submitted Mar 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"