The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa).
Use at 2-5 µg/mg of lysate.
FunctionBinds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
Tissue specificityHighest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
Sequence similaritiesBelongs to the ubiquitin conjugation factor E4 family. Contains 1 U-box domain.
Post-translational modificationsProteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
All lanes : Anti-UBE4B antibody (ab72497) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 146 kDa Observed band size : 146 kDa Additional bands at : 117 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Immunoprecipitation - UBE4B antibody (ab72497)
Detection of UBE4B by Western Blot of Immunprecipitate.
ab72497 at 0.1µg/ml staining UBE4B in HeLa whole cell lysate immunoprecipitated using ab72497 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes.
References for Anti-UBE4B antibody (ab72497)
has not yet been referenced specifically in any publications.
Publishing research using ab72497? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"