Overview

  • Product nameAnti-UBE4B antibody
    See all UBE4B primary antibodies
  • Description
    Rabbit polyclonal to UBE4B
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 125 and 175 of human UBE4B (AAD02233.1)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab72497 was affinity purified using an epitope specific to UBE4B immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionBinds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
  • Tissue specificityHighest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
  • Sequence similaritiesBelongs to the ubiquitin conjugation factor E4 family.
    Contains 1 U-box domain.
  • Post-translational
    modifications
    Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930551I19Rik antibody
    • 4933406G05Rik antibody
    • AU014668 antibody
    • D4Bwg0973e antibody
    • E4 antibody
    • HDNB1 antibody
    • Homozygously deleted in neuroblastoma 1 antibody
    • KIAA0684 antibody
    • mKIAA0684 antibody
    • RP23 173D8.1 antibody
    • Ube4b antibody
    • UBE4B_HUMAN antibody
    • Ubiquitin conjugation factor E4 B antibody
    • Ubiquitin fusion degradation protein 2 antibody
    • UBOX3 antibody
    • UFD2 antibody
    • UFD2a antibody
    • Ufd2p antibody
    see all

Anti-UBE4B antibody images

  • All lanes : Anti-UBE4B antibody (ab72497) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 146 kDa
    Observed band size : 146 kDa
    Additional bands at : 117 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of UBE4B by Western Blot of Immunprecipitate.
    ab72497 at 0.1µg/ml staining UBE4B in HeLa whole cell lysate immunoprecipitated using ab72497 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 minutes.

References for Anti-UBE4B antibody (ab72497)

ab72497 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human skin fibroblasts and U2OS)
Specification Human skin fibroblasts and U2OS
Fixative Formaldehyde
Permeabilization Yes - 0.5% triton 10min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"