Overview

  • Product name
  • Description
    Rabbit polyclonal to UBE4B
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1252 and 1302 of human UBE4B (AAD02233.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab72501 was affinity purified using an epitope specific to UBE4B immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
    • Tissue specificity
      Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
    • Sequence similarities
      Belongs to the ubiquitin conjugation factor E4 family.
      Contains 1 U-box domain.
    • Post-translational
      modifications
      Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930551I19Rik antibody
      • 4933406G05Rik antibody
      • AU014668 antibody
      • D4Bwg0973e antibody
      • E4 antibody
      • HDNB1 antibody
      • Homozygously deleted in neuroblastoma 1 antibody
      • KIAA0684 antibody
      • mKIAA0684 antibody
      • RP23 173D8.1 antibody
      • Ube4b antibody
      • UBE4B_HUMAN antibody
      • Ubiquitin conjugation factor E4 B antibody
      • Ubiquitin fusion degradation protein 2 antibody
      • UBOX3 antibody
      • UFD2 antibody
      • UFD2a antibody
      • Ufd2p antibody
      see all

    Images

    • Detection of UBE4B by Western Blot of Immunprecipitate.
      ab72497 at 0.1µg/ml staining UBE4B in HeLa whole cell lysate immunoprecipitated using ab72501 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 minutes.

    References

    ab72501 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Specification
    U2OS
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton 0.5%
    Username

    Abcam user community

    Verified customer

    Submitted Dec 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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