Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
Belongs to the ubiquitin conjugation factor E4 family. Contains 1 U-box domain.
Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
Western blot - Anti-UBE4B antibody [EPR7471] (ab126759)
All lanes : Anti-UBE4B antibody [EPR7471] (ab126759) at 1/1000 dilution
Lane 1 : 293T cell lysate Lane 2 : Raji cell lysate Lane 3 : SK OV 3 cell lysate Lane 4 : L929 cell lysate Lane 5 : L6 cell lysate Lane 6 : RAW264.7 cell lysate Lane 7 : PC12 cell lysate Lane 8 : NIH 3T3 cell lysate Lane 9 : HeLa cell lysate Lane 10 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 146 kDa
Western blot - Anti-UBE4B antibody [EPR7471] (ab126759)This image is courtesy of an anonymous Abreview
Western blot analysis of Human 293NT whole cell lysate labeling UBE4B with ab126759 at 1/2000. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/20000) was used as the secondary antibody.
siRNA agains UBE4B was used to show for antibody specificity.
Unspecific siRNA (siLUC) was used as a negative control.