Recombinant
RabMAb

Anti-UBE4B antibody [EPR7471] (ab126759)

Overview

  • Product name
    Anti-UBE4B antibody [EPR7471]
    See all UBE4B primary antibodies
  • Description
    Rabbit monoclonal [EPR7471] to UBE4B
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human UBE4B.

  • Positive control
    • 293T, Raji, SK OV 3, L929, L6, RAW264.7, PC12, NIH 3T3, HeLa, and K562 cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7471
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
    • Tissue specificity
      Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
    • Sequence similarities
      Belongs to the ubiquitin conjugation factor E4 family.
      Contains 1 U-box domain.
    • Post-translational
      modifications
      Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930551I19Rik antibody
      • 4933406G05Rik antibody
      • AU014668 antibody
      • D4Bwg0973e antibody
      • E4 antibody
      • HDNB1 antibody
      • Homozygously deleted in neuroblastoma 1 antibody
      • KIAA0684 antibody
      • mKIAA0684 antibody
      • RP23 173D8.1 antibody
      • Ube4b antibody
      • UBE4B_HUMAN antibody
      • Ubiquitin conjugation factor E4 B antibody
      • Ubiquitin fusion degradation protein 2 antibody
      • UBOX3 antibody
      • UFD2 antibody
      • UFD2a antibody
      • Ufd2p antibody
      see all

    Images

    • All lanes : Anti-UBE4B antibody [EPR7471] (ab126759) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : SK OV 3 cell lysate
      Lane 4 : L929 cell lysate
      Lane 5 : L6 cell lysate
      Lane 6 : RAW264.7 cell lysate
      Lane 7 : PC12 cell lysate
      Lane 8 : NIH 3T3 cell lysate
      Lane 9 : HeLa cell lysate
      Lane 10 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 146 kDa

    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 146 kDa


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      Western blot analysis of Human 293NT whole cell lysate labeling UBE4B with ab126759 at 1/2000. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/20000) was used as the secondary antibody.

      siRNA agains UBE4B was used to show for antibody specificity.

      Unspecific siRNA (siLUC) was used as a negative control.

      See Abreview

    References

    ab126759 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (u2os)
    Specification
    u2os
    Permeabilization
    Yes - 0.5% triton
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 08 2014

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (293NT)
    Specification
    293NT
    Username

    Abcam user community

    Verified customer

    Submitted Jan 08 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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