Overview

  • Product name
  • Description
    Mouse monoclonal to UBF1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cynomolgus monkey, Chinese hamster
  • Immunogen

    Recombinant fragment with tag: PPAATNSSKK MKFQGEPKKP PMNGYQKFSQ ELLSNGELNH LPLKERMVEI GSRWQRISQS QKEHYKKLAE EQQKQYKVHL DLWVKSLSPQ DRAAYKEYIS , corresponding to amino acids 551-651 of Human UBF1

  • Positive control
    • HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab61205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function
    Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
  • Sequence similarities
    Contains 6 HMG box DNA-binding domains.
  • Post-translational
    modifications
    Phosphorylated and activated by PIK3CA.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa nucleolus organizer region autoantigen antibody
    • Autoantigen NOR-90 antibody
    • NOR 90 antibody
    • Nucleolar transcription factor 1 antibody
    • UBF 1 antibody
    • UBF antibody
    • UBF-1 antibody
    • UBF1_HUMAN antibody
    • Ubtf antibody
    • Upstream binding factor 1 antibody
    • upstream binding transcription factor, RNA polymerase I antibody
    • Upstream-binding factor 1 antibody
    see all

Anti-UBF1 antibody images

  • Anti-UBF1 antibody (ab61205) at 1 µg/ml + HepG2 whole cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa

References for Anti-UBF1 antibody (ab61205)

ab61205 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab61205.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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