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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
1/50 - 1/100.
Function Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Sequence similarities Contains 6 HMG box DNA-binding domains.
Post-translational modifications Phosphorylated and activated by PIK3CA.
Cellular localization Nucleus > nucleolus.
Information by UniProt
90 kDa nucleolus organizer region autoantigen antibody
Autoantigen NOR-90 antibody
NOR 90 antibody
Anti-UBF1 antibody images
Western blot - UBF1 antibody (ab65059)
All lanes : Anti-UBF1 antibody (ab65059) at 1/500 dilution Lane 1 : Extracts from HeLa cells at 5-30ug Lane 2 : Extracts from Jurkat cells at 5-30ug Lane 3 : Extracts from Jurkat cells at 5-30ug with immunizing peptide at 5-10ug Predicted band size : 89 kDa Observed band size : 89 kDa Additional bands at : 45 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBF1 antibody (ab65059)
Immunohistochemistry analysis of paraffin-embedded
human brain tissue using ab65059 at a dilution of 1/50-1/100. Left hand panel: without immunizing peptide. Right hand panel: with immunizing peptide.
References for Anti-UBF1 antibody (ab65059)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"