Overview

  • Product nameAnti-UBF1 antibodySee all UBF1 primary antibodies ...
  • Description
    Rabbit polyclonal to UBF1
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human UBF1.

Properties

Applications

Our Abpromise guarantee covers the use of ab65059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • FunctionRecognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
  • Sequence similaritiesContains 6 HMG box DNA-binding domains.
  • Post-translational
    modifications
    Phosphorylated and activated by PIK3CA.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 90 kDa nucleolus organizer region autoantigen antibody
    • Autoantigen NOR-90 antibody
    • NOR 90 antibody
    • Nucleolar transcription factor 1 antibody
    • UBF 1 antibody
    • UBF antibody
    • UBF-1 antibody
    • UBF1_HUMAN antibody
    • UBTF antibody
    • Upstream binding factor 1 antibody
    • upstream binding transcription factor, RNA polymerase I antibody
    • Upstream-binding factor 1 antibody
    see all

Anti-UBF1 antibody images

  • All lanes : Anti-UBF1 antibody (ab65059) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells at 5-30ug
    Lane 2 : Extracts from Jurkat cells at 5-30ug
    Lane 3 : Extracts from Jurkat cells at 5-30ug with immunizing peptide at 5-10ug


    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 45 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab65059 at a dilution of 1/50-1/100. Left hand panel: without immunizing peptide. Right hand panel: with immunizing peptide.

References for Anti-UBF1 antibody (ab65059)

ab65059 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Osteosarcoma SaOS-2)
Specification Osteosarcoma SaOS-2
Fixative Paraformaldehyde
Permeabilization Yes - PBS TRITON 0.25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Submitted May 29 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"