Overview

  • Product name
    Anti-UBF1 antibody [EP2741Y]
    See all UBF1 primary antibodies
  • Description
    Rabbit monoclonal [EP2741Y] to UBF1
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human UBF1

  • Positive control
    • HeLa and A431 cell lysates, human breast carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/30 - 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
    • Sequence similarities
      Contains 6 HMG box DNA-binding domains.
    • Post-translational
      modifications
      Phosphorylated and activated by PIK3CA.
    • Cellular localization
      Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa nucleolus organizer region autoantigen antibody
      • Autoantigen NOR-90 antibody
      • NOR 90 antibody
      • Nucleolar transcription factor 1 antibody
      • UBF 1 antibody
      • UBF antibody
      • UBF-1 antibody
      • UBF1_HUMAN antibody
      • Ubtf antibody
      • Upstream binding factor 1 antibody
      • upstream binding transcription factor, RNA polymerase I antibody
      • Upstream-binding factor 1 antibody
      see all

    Images

    • Overlay histogram showing A431 cells stained with ab75781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75781, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-UBF1 antibody [EP2741Y] (ab75781) at 1/500 dilution

      Lane 1 : (A) HeLa cell lysate
      Lane 2 : (B) A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 89 kDa
      Observed band size : 89 kDa
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab75781 at 1/100-1/250 dilution.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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