Overview

  • Product nameAnti-UBF1 antibody [EP2741Y]
    See all UBF1 primary antibodies
  • Description
    Rabbit monoclonal [EP2741Y] to UBF1
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human UBF1

  • Positive control
    • HeLa and A431 cell lysates, human breast carcinoma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab75781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/30 - 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionRecognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
    • Sequence similaritiesContains 6 HMG box DNA-binding domains.
    • Post-translational
      modifications
      Phosphorylated and activated by PIK3CA.
    • Cellular localizationNucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa nucleolus organizer region autoantigen antibody
      • Autoantigen NOR-90 antibody
      • NOR 90 antibody
      • Nucleolar transcription factor 1 antibody
      • UBF 1 antibody
      • UBF antibody
      • UBF-1 antibody
      • UBF1_HUMAN antibody
      • Ubtf antibody
      • Upstream binding factor 1 antibody
      • upstream binding transcription factor, RNA polymerase I antibody
      • Upstream-binding factor 1 antibody
      see all

    Anti-UBF1 antibody [EP2741Y] images

    • Overlay histogram showing A431 cells stained with ab75781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75781, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-UBF1 antibody [EP2741Y] (ab75781) at 1/500 dilution

      Lane 1 : (A) HeLa cell lysate
      Lane 2 : (B) A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 89 kDa
      Observed band size : 89 kDa
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab75781 at 1/100-1/250 dilution.

    References for Anti-UBF1 antibody [EP2741Y] (ab75781)

    ab75781 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab75781.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"