Overview

  • Product nameAnti-UBF1 antibody [EPR2742]
    See all UBF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2742] to UBF1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human UBF1.

  • Positive control
    • HeLa, A431, MCF7 and JAR cell lysates; Human colon and breast tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionRecognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
    • Sequence similaritiesContains 6 HMG box DNA-binding domains.
    • Post-translational
      modifications
      Phosphorylated and activated by PIK3CA.
    • Cellular localizationNucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 90 kDa nucleolus organizer region autoantigen antibody
      • Autoantigen NOR-90 antibody
      • NOR 90 antibody
      • Nucleolar transcription factor 1 antibody
      • UBF 1 antibody
      • UBF antibody
      • UBF-1 antibody
      • UBF1_HUMAN antibody
      • Ubtf antibody
      • Upstream binding factor 1 antibody
      • upstream binding transcription factor, RNA polymerase I antibody
      • Upstream-binding factor 1 antibody
      see all

    Anti-UBF1 antibody [EPR2742] images

    • All lanes : Anti-UBF1 antibody [EPR2742] (ab109011) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 89 kDa
    • ab109011 at 1/50 dilution staining UBF1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109011 at 1/50 dilution staining UBF1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue

    References for Anti-UBF1 antibody [EPR2742] (ab109011)

    ab109011 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109011.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"