Overview

  • Product nameAnti-Ubiquilin antibody
    See all Ubiquilin primary antibodies
  • Description
    Rabbit polyclonal to Ubiquilin
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Ubiquilin aa 2-18.
    Sequence:

    AESAESGGPPGAQDSAA

  • Positive control
    • CHO cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract.

IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml. PubMed: 19112176

Target

  • FunctionLinks CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors (By similarity). Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation.
  • Tissue specificityUbiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney.
  • Sequence similaritiesContains 1 UBA domain.
    Contains 1 ubiquitin-like domain.
  • DomainBinds PSEN1 and PSEN2 via its UBA domain.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DA41 antibody
    • DSK2 antibody
    • FLJ90054 antibody
    • hPLIC-1 antibody
    • hPLIC1 antibody
    • PLIC-1 antibody
    • PLIC1 antibody
    • Protein linking IAP with cytoskeleton 1 antibody
    • Ubiquilin-1 antibody
    • Ubiquilin1 antibody
    • UBQL1_HUMAN antibody
    • UBQLN1 antibody
    • UBQN antibody
    • XDRP1 antibody
    see all

Anti-Ubiquilin antibody images



  • Predicted band size : 63 kDa

    Western blot detection of Ubiquilin on CHO cell extract using ab3341.

References for Anti-Ubiquilin antibody (ab3341)

This product has been referenced in:
  • Adusei DC  et al. Early developmental alterations in GABAergic protein expression in fragile X knockout mice. Neuropharmacology 59:167-71 (2010). WB ; Mouse . Read more (PubMed: 20470805) »
  • Hiltunen M  et al. Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion. J Biol Chem 281:32240-53 (2006). ICC/IF ; Mouse . Read more (PubMed: 16945923) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (U2OS)
Specification U2OS
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Randal Tibbetts

Verified customer

Submitted May 23 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"