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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
Overview
Product name
Anti-Ubiquitin antibody
See all Ubiquitin products (31) ...
Description
Rabbit polyclonal to Ubiquitin
Specificity
It can identify free ubiquitin as well as ubiquinated proteins.
Tested applications
Immunoelectrophoresis, IHC-FoFr, IHC-P, ICC/IF, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Xenopus laevis, Fruit fly (Drosophila melanogaster), Fish, Monkey, Escherichia coli
Immunogen
Full length native protein: Ubiquitin purified from bovine red blood cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, Whole serum, PBS, pH 7.2
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Tangles & Tau
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ubiquitin antibody (ab19247)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab19247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunoelectrophoresis
Ie: 1/1000Ie: 1/1000
IHC-FoFr
IHC-FoFr: 1/200IHC-FoFr: 1/200
IHC-P: Use at an assay dependent dilution. (PubMed: 20876612)
ICC/IF: Use at an assay dependent dilution.
WB: 1/1000Predicted molecular weight: 10 kDa.
IP: 1/100
Target
Relevance
Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
Cellular localization
Cell Membrane, Cytoplasmic and Nuclear
Alternative names
- FLJ25987 antibody
- MGC8385 antibody
- Polyubiquitin B antibody
see all
Database links
- Entrez Gene: 396190 Chicken
- Entrez Gene: 281370 Cow
- Entrez Gene: 479513 Dog
- Entrez Gene: 7314 Human
- Entrez Gene: 22187 Mouse
- Entrez Gene: 100125968 Pig
- Entrez Gene: 192255 Rat
- Omim: 191339 Human
see all
Anti-Ubiquitin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ubiquitin antibody (ab19247)
Ab19247 staining Human normal testis. Staining is localised to the nucleus and cytoplasm.
Left panel: with primary antibody diluted at 1:1000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Ubiquitin antibody (ab19247)
This product has been referenced in:
- Azzu V & Brand MD Degradation of an intramitochondrial protein by the cytosolic proteasome. J Cell Sci 123:578-85 (2010). IP.Read more (PubMed: 20103532) »
- Polito VAet al. Correction of CNS defects in the MPSII mouse model via systemic enzyme replacement therapy. Hum Mol Genet 19:4871-85 (2010). IHC-P; Mouse.Read more (PubMed: 20876612) »
See all 6 publications for this product
Publishing research using ab19247? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"