Overview

  • Product nameAnti-Ubiquitin antibody
    See all Ubiquitin primary antibodies
  • Description
    Sheep polyclonal to Ubiquitin
  • SpecificityRecognises both free ubiquitin and ubiquitin protein conjugates on western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections.
  • Tested applicationsSuitable for: Dot blot, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Human
  • Immunogen

    Ubiquitin from Sigma (Product number U6253) that was coupled to chicken gammaglobulin.

Properties

Applications

Our Abpromise guarantee covers the use of ab6181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
IHC-Fr
WB
  • Application notesImmunohistochemistry: 1/2,000 to 5,000
    Western Blot: 1/1,000 to 2,000
    Immunoblot: 1/1,000 to 2,000
    1 site ELISA: Not tested.
    Biological Inhibition: Not tested.
  • Target

    • RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localizationCell Membrane, Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • FLJ25987 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all

    References for Anti-Ubiquitin antibody (ab6181)

    ab6181 has not yet been referenced specifically in any publications.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"