• Product nameAnti-Ubiquitin antibody
    See all Ubiquitin primary antibodies
  • Description
    Rabbit polyclonal to Ubiquitin
  • SpecificityRecognises 1-3 bands in Western blot with bovine red blood cell ubiquitin.
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Saccharomyces cerevisiae
    Predicted to work with: a wide range of other species
  • Immunogen

    Tissue, cells or virus corresponding to Cow Ubiquitin conjugated to Keyhole Limpet Haemocyanin (KLH). Bovine red blood cell ubiquitin



Our Abpromise guarantee covers the use of ab6309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-Fr: Use at an assay dependent concentration.
    WB: Use at an assay dependent concentration.
    Suggested working dilution(s): Western blotting - minimum 1/100 (purified ubiquitin from bovine RBC)
    This antibody gives a nice positive signal on 5 ug of yeast ubiquitin. It is not as strong as the signal with bovine but is still detectable.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localizationCell Membrane, Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • FLJ25987 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all

    Anti-Ubiquitin antibody images

    • Immunofluorescence of P23H mutant cells with ubiquitin, showing co-localisation of mutant aggregated protein with ubiquitin (1 / 500 dilution). This picture was kindly supplied as part of the review submitted by Ritu Malhorta.

    References for Anti-Ubiquitin antibody (ab6309)

    This product has been referenced in:
    • Zvirbliene A  et al. The use of chimeric virus-like particles harbouring a segment of hantavirus Gc glycoprotein to generate a broadly-reactive hantavirus-specific monoclonal antibody. Viruses 6:640-60 (2014). Read more (PubMed: 24513568) »
    • Howarth J  et al. Hsp40 Molecules That Target to the Ubiquitin-proteasome System Decrease Inclusion Formation in Models of Polyglutamine Disease. Mol Ther 15:1100-5 (2007). WB ; Mouse . Read more (PubMed: 17426712) »

    See all 2 Publications for this product

    Product Wall

    This antibody has not yet been tested against yeast proteins.