Overview

  • Product nameAnti-Ubiquitin antibody [EP296Y]
    See all Ubiquitin primary antibodies
  • Description
    Rabbit monoclonal [EP296Y] to Ubiquitin
  • SpecificityThe antibody recognizes only mono-ubiquitinated molecules.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminal of Human Ubiquitin.

  • Positive control
    • Hela cell lysate and human prostate adenocarcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab33893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 8.5 kDa (predicted molecular weight: 8 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localizationCell Membrane, Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • FLJ25987 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all

    Anti-Ubiquitin antibody [EP296Y] images

    References for Anti-Ubiquitin antibody [EP296Y] (ab33893)

    This product has been referenced in:
    • Sidoryk-Wegrzynowicz M  et al. Disruption of astrocytic glutamine turnover by manganese is mediated by the protein kinase C pathway. Glia 59:1732-43 (2011). WB ; Human . Read more (PubMed: 21812036) »
    • Liu G & Xirodimas DP NUB1 promotes cytoplasmic localization of p53 through cooperation of the NEDD8 and ubiquitin pathways. Oncogene : (2010). WB . Read more (PubMed: 20101219) »

    See all 5 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Mar 27 2014

    All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention.

    As ICC/IF is not listed as a tested application, we ...

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    Merci pour votre appel de hier et votre patience.

    J'ai regardé nos anticorps contre l'ubiquitine en un peu plus de détails. La décision n'est pas très facile. Voici mes recommandations/commentaires:

    Le ab33893 est un anticorps mon...

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    Thank you for your enquiry. This antibody does indeed cross-react with mouse and rat, in addition to human. I am not sure why the specificity statment was on the datasheet, but I have removed it to avoid future confusion. I hope this informatio...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"