Recombinant
RabMAb

Anti-Ubiquitin antibody [EP296Y] (ab33893)

Overview

  • Product name
    Anti-Ubiquitin antibody [EP296Y]
    See all Ubiquitin primary antibodies
  • Description
    Rabbit monoclonal [EP296Y] to Ubiquitin
  • Specificity
    The antibody recognizes only mono-ubiquitinated molecules.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminal of Human Ubiquitin.

  • Positive control
    • Hela cell lysate and human prostate adenocarcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab33893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 8.5 kDa (predicted molecular weight: 8 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Relevance
      Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localization
      Cell Membrane, Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Epididymis secretory protein Li 50 antibody
      • FLJ25987 antibody
      • HEL S 50 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all

    Anti-Ubiquitin antibody [EP296Y] images

    References for Anti-Ubiquitin antibody [EP296Y] (ab33893)

    This product has been referenced in:
    • He Y  et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). Read more (PubMed: 27959342) »
    • Sidoryk-Wegrzynowicz M  et al. Disruption of astrocytic glutamine turnover by manganese is mediated by the protein kinase C pathway. Glia 59:1732-43 (2011). WB ; Human . Read more (PubMed: 21812036) »

    See all 6 Publications for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Mar 27 2014

    All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention.

    As ICC/IF is not listed as a tested application, we ...

    Read More

    Merci pour votre appel de hier et votre patience.

    J'ai regardé nos anticorps contre l'ubiquitine en un peu plus de détails. La décision n'est pas très facile. Voici mes recommandations/commentaires:

    Le ab33893 est un anticorps mon...

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    Thank you for your enquiry. This antibody does indeed cross-react with mouse and rat, in addition to human. I am not sure why the specificity statment was on the datasheet, but I have removed it to avoid future confusion. I hope this informatio...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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