The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: 1/1000. Detects a band of approximately 6.5 kDa (predicted molecular weight: 9 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Similarity: Belongs to the ubiquitin family.
Contains 3 ubiquitin-like domains.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
Western blot - Anti-Ubiquitin antibody [P4G7-H11] (ab90376)
All lanes : Anti-Ubiquitin antibody [P4G7-H11] (ab90376) at 1/1000 dilution
Lane 1 : Molecular weight marker Lane 2 : Ubiquitin Lane 3 : HeLa cell lysate Lane 4 : RK-13 cell lysate Lane 5 : 3T3 cell lysate Lane 6 : PC12 cell lysate Lane 7 : Mouse brain tissue extract Lane 8 : Rat brain tissue extract Lane 9 : MDBK cell lysate
Predicted band size : 9 kDa
References for Anti-Ubiquitin antibody [P4G7-H11] (ab90376)
This product has been referenced in:
Szilagyi Z et al. Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast. Mol Cell Biol32:2099-109 (2012).
Read more (PubMed: 22451489) »