Overview

  • Product nameAnti-Ubiquitin antibody [P4G7-H11]
    See all Ubiquitin primary antibodies
  • Description
    Mouse monoclonal [P4G7-H11] to Ubiquitin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Human, Schizosaccharomyces pombe
  • Immunogen

    Native bovine Ubiquitin

  • Positive control
    • HeLa, RK-13, 3T3, PC12 and MDBK cell lysates; Mouse and Rat brain tissue extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab90376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Detects a band of approximately 6.5 kDa (predicted molecular weight: 9 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localizationCell Membrane, Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • FLJ25987 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all

    Anti-Ubiquitin antibody [P4G7-H11] images

    • All lanes : Anti-Ubiquitin antibody [P4G7-H11] (ab90376) at 1/1000 dilution

      Lane 1 : Molecular weight marker
      Lane 2 : Ubiquitin
      Lane 3 : HeLa cell lysate
      Lane 4 : RK-13 cell lysate
      Lane 5 : 3T3 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : Mouse brain tissue extract
      Lane 8 : Rat brain tissue extract
      Lane 9 : MDBK cell lysate


      Predicted band size : 9 kDa

    References for Anti-Ubiquitin antibody [P4G7-H11] (ab90376)

    This product has been referenced in:
    • Szilagyi Z  et al. Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast. Mol Cell Biol 32:2099-109 (2012). WB ; Schizosaccharomyces pombe . Read more (PubMed: 22451489) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"