Overview

  • Product nameAnti-UBL7 antibody
    See all UBL7 primary antibodies
  • Description
    Rabbit polyclonal to UBL7
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide, corresponding to a region wtihin amino acids 330-380 of Human UBL7 (NP_116296.1).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificityUbiquitous. Highly expressed in heart, skeletal muscle, testis, thyroid and adrenal gland.
  • Sequence similaritiesContains 1 UBA domain.
    Contains 1 ubiquitin-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2300004C15Rik antibody
    • BMSC UbP antibody
    • BMSC-UbP antibody
    • BMSCUBP antibody
    • Bone marrow stromal cell derived ubiquitin like protein antibody
    • Bone marrow stromal cell ubiquitin-like protein antibody
    • MGC14421 antibody
    • MGC94284 antibody
    • SB132 antibody
    • TCBA1 antibody
    • Ubiquitin like 7 (bone marrow stromal cell derived) antibody
    • Ubiquitin like 7 antibody
    • Ubiquitin like protein SB132 antibody
    • Ubiquitin-like protein 7 antibody
    • Ubiquitin-like protein SB132 antibody
    • Ubl7 antibody
    • UBL7_HUMAN antibody
    see all

Anti-UBL7 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling UBL7 with ab115041 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling UBL7 with ab115041 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-UBL7 antibody (ab115041) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 30 seconds
  • Detection of UBL7 by Western Blot of Immunprecipitates.
    ab115041 at 1µg/ml staining UBL7 in HeLa whole cell lysate immunoprecipitated using ab115041 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-UBL7 antibody (ab115041)

ab115041 has not yet been referenced specifically in any publications.

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