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Our Abpromise guarantee covers the use of ab157043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
TECHNICAL NOTE: Under certain conditions these polyubiquitinylated substrates can bind to metal affinity matrices.
USES: Deubiquitinylating enzyme substrates (general/linkage specific), Profiling of DUB linkage-type preference or specificity in combination with other single lysine only polyubiquitinylated substrates (e.g. (K11-only)Ubn-ubiquitinylated substrate), investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.02% DTT, 0.605% Tris, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Lane 1: MW Marker, Lane 2: 2 μg of ab157043, Lane 3: 5 μg of ab157043, Lane 4: 10 μg of ab157043
DUB assay. ab157043 was incubated with USP2. Lane 1: MW Marker, Lane 2: Substrate without protease, Lane 3: Substrate with protease, Lane 4: Protease without substrate.
ab157043 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"