The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 122 kDa).
Use a concentration of 1 - 5 µg/ml.
RelevanceUBN1 may be required for replication independent chromatin assembly, and interacts with epstein-barr virus BZLF1 and CEBPA. It is ubiquitous, and is also expressed in numerous tumours and cancer cell lines. Interacts with epstein-barr virus BZLF1 and CEBPA.
Overlay histogram showing HeLa cells stained with ab84953 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84953, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG ( 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - UBN1 antibody [UBN1-02] (ab84953)
Lane 1 : Anti-UBN1 antibody [UBN1-02] (ab84953) at 1 µg/ml Lane 2 : Anti-UBN1 antibody [UBN1-02] (ab84953) at 5 µg/ml
Lane 1 : HeLa nuclear lysate Lane 2 : HeLa nuclear lysate