Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
1/10000 - 1/50000.
FunctionCan efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
Sequence similaritiesBelongs to the peptidase C19 family.
ab115618, at 5 µg/ml, staining UBP43 in Formalin-fixed, Paraffin-embedded Human heart tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-UBP43 antibody (ab115618)
has not yet been referenced specifically in any publications.
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