Overview

  • Product name
  • Description
    Rabbit polyclonal to UBP43
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide: EKMQDSRQKA VRPLELAYCL QKCNVPLFVQ HDAAQLYLKL WNLIKDQITD , corresponding to N terminal amino acids 108 - 157 of Human UBP43

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
  • Sequence similarities
    Belongs to the peptidase C19 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 43 kDa ISG15 specific protease antibody
    • 43 kDa ISG15-specific protease antibody
    • EC 3.1.2. antibody
    • hUBP43 antibody
    • Interferon Stimulated Gene 43 kD antibody
    • ISG15 Specific Processing Protease antibody
    • ISG15-specific-processing protease antibody
    • ISG43 antibody
    • Ubiquitin Specific Peptidase 18 antibody
    • Ubiquitin Specific Protease 18 43 kD antibody
    • Ubiquitin Specific Protease 18 antibody
    • Ubl carboxyl terminal hydrolase 18 antibody
    • Ubl carboxyl-terminal hydrolase 18 antibody
    • Ubl thioesterase 18 antibody
    • Ubl thiolesterase 18 antibody
    • Ubp15 antibody
    • UBP18_HUMAN antibody
    • USP18 antibody
    see all

Images

  • Anti-UBP43 antibody (ab80815) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

References

ab80815 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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