Overview

  • Product nameAnti-Ubp64E antibody
    See all Ubp64E primary antibodies
  • Description
    Mouse polyclonal to Ubp64E
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide:

    LPDLYKPRVYFYNPSLKKMK

    , corresponding to amino acids 485-504 of Fruit fly (Drosophila melanogaster) Ubp64E

  • General notes

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 32.2 kDa (predicted molecular weight: 174 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • RelevanceUbp64E is a ubiquitin specific protease, and has an effect on position effect variegation. It belongs to the peptidase C19 family.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • Deubiquitinating enzyme 64E antibody
      • Ubiquitin specific protease 64E antibody
      • Ubiquitin thiolesterase 64E antibody
      • UBP antibody
      • Ubp64 antibody
      see all

    Anti-Ubp64E antibody images

    • All lanes : Anti-Ubp64E antibody (ab52688) at 1/1000 dilution

      Lane 1 : The negative control lane: ~20ug of a total protein extract from E coli with ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen.
      Lane 2 : Test lane: ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein)

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 174 kDa
      Observed band size : 32.2 kDa (why is the actual band size different from the predicted?)

    References for Anti-Ubp64E antibody (ab52688)

    ab52688 has not yet been referenced specifically in any publications.

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