Overview

  • Product nameAnti-UBPY/USP8 antibody
    See all UBPY/USP8 primary antibodies
  • Description
    Rabbit polyclonal to UBPY/USP8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 861-910 (FAGYSQQDSQ ELLLFLMDGL HEDLNKADNR KRYKEENNDH LDDFKAAEHA) of Human UBPY/USP8 (NP_005145).

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab108102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 128 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
  • Sequence similaritiesBelongs to the peptidase C19 family.
    Contains 1 MIT domain.
    Contains 1 rhodanese domain.
    Contains 1 USP domain.
  • DomainThe MIT domain is required for endosomal localization, CHMP1B-binding, maintenance of ESCRT-0 stability and EGFR degradation.
    The rhodanese domain is sufficient for RNF41-binding.
  • Post-translational
    modifications
    Phosphorylation of Ser-718 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-718 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner.
    Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function.
  • Cellular localizationCytoplasm. Nucleus. Endosome membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 8 antibody
    • FLJ34456 antibody
    • hUBPy antibody
    • HumORF8 antibody
    • KIAA0055 antibody
    • MGC129718 antibody
    • Ubiquitin carboxyl-terminal hydrolase 8 antibody
    • Ubiquitin isopeptidase Y antibody
    • ubiquitin specific peptidase 8 antibody
    • Ubiquitin thioesterase 8 antibody
    • Ubiquitin-specific-processing protease 8 antibody
    • UBP8_HUMAN antibody
    • UBPY antibody
    • Usp8 antibody
    see all

Anti-UBPY/USP8 antibody images

  • Anti-UBPY/USP8 antibody (ab108102) at 1 µg/ml + 293T cell lysate at 10 µg

    Predicted band size : 128 kDa

References for Anti-UBPY/USP8 antibody (ab108102)

ab108102 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab108102.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"