• Product nameAnti-UBPY/USP8 antibody
    See all UBPY/USP8 primary antibodies
  • Description
    Rabbit polyclonal to UBPY/USP8
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 50 aa of human USP8.

  • Positive control
    • Transfected 293T cells.



Our Abpromise guarantee covers the use of ab59949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).


  • FunctionHydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
  • Sequence similaritiesBelongs to the peptidase C19 family.
    Contains 1 MIT domain.
    Contains 1 rhodanese domain.
    Contains 1 USP domain.
  • DomainThe MIT domain is required for endosomal localization, CHMP1B-binding, maintenance of ESCRT-0 stability and EGFR degradation.
    The rhodanese domain is sufficient for RNF41-binding.
  • Post-translational
    Phosphorylation of Ser-718 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-718 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner.
    Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function.
  • Cellular localizationCytoplasm. Nucleus. Endosome membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deubiquitinating enzyme 8 antibody
    • FLJ34456 antibody
    • hUBPy antibody
    • HumORF8 antibody
    • KIAA0055 antibody
    • MGC129718 antibody
    • Ubiquitin carboxyl-terminal hydrolase 8 antibody
    • Ubiquitin isopeptidase Y antibody
    • ubiquitin specific peptidase 8 antibody
    • Ubiquitin thioesterase 8 antibody
    • Ubiquitin-specific-processing protease 8 antibody
    • UBP8_HUMAN antibody
    • UBPY antibody
    • Usp8 antibody
    see all

Anti-UBPY/USP8 antibody images

  • Anti-UBPY/USP8 antibody (ab59949) at 1/100 dilution + Transfected 293T cells

    Predicted band size : 128 kDa
    Observed band size : 128 kDa

References for Anti-UBPY/USP8 antibody (ab59949)

This product has been referenced in:
  • Panner A  et al. Ubiquitin-specific protease 8 links the PTEN-Akt-AIP4 pathway to the control of FLIPS stability and TRAIL sensitivity in glioblastoma multiforme. Cancer Res 70:5046-53 (2010). WB ; Human, Mouse . Read more (PubMed: 20484045) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Sep 07 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets

Abcam user community

Verified customer

Submitted Dec 21 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (PMN)
Specification PMN
Fixative Formaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 2%

Abcam user community

Verified customer

Submitted Feb 18 2010

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 5 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12.5 % gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 14 2009