Anti-UBR1 antibody (ab108215)
Key features and details
- Rabbit polyclonal to UBR1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBR1 antibody
See all UBR1 primary antibodies -
Description
Rabbit polyclonal to UBR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to a region between amino acids 1125-1175 of Human UBR1 (NP_777576.1).
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Positive control
- Jurkat whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab108215 was affinity purified using an epitope specific to UBR1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 200 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 200 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. -
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in UBR1 are a cause of Johanson-Blizzard syndrome (JBS) [MIM:243800]. This disorder includes congenital exocrine pancreatic insufficiency, multiple malformations such as nasal wing aplasia, and frequent mental retardation. Pancreas of individuals with JBS do not express UBR1 and show intrauterine-onset destructive pancreatitis. -
Sequence similarities
Belongs to the UBR1 family.
Contains 1 RING-type zinc finger.
Contains 1 UBR-type zinc finger. -
Developmental stage
Expressed in fetal pancreas. -
Domain
The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 197131 Human
- Omim: 605981 Human
- SwissProt: Q8IWV7 Human
- Unigene: 591121 Human
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Alternative names
- E3 ubiquitin-protein ligase UBR1 antibody
- JBS antibody
- N-recognin-1 antibody
see all
Images
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All lanes : Anti-UBR1 antibody (ab108215) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 200 kDa
Exposure time: 3 minutes -
Detection of UBR1 by Western Blot of Immunprecipitate.
Lane 1: ab108215 at 1µg/ml staining UBR1 in Jurkat whole cell lysate immunoprecipitated using ab108215 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab108215 has not yet been referenced specifically in any publications.