• Product name
  • Description
    Rabbit polyclonal to UBR1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human UBR1.

    (Peptide available as ab42419.)

  • Positive control
    • Skeletal Muscle (Human) Tissue Lysate - adult normal tissue HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate HeLa (Human epithelial carcinoma cell line) Nuclear Lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab42420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).


  • Function
    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth.
  • Tissue specificity
    Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Defects in UBR1 are a cause of Johanson-Blizzard syndrome (JBS) [MIM:243800]. This disorder includes congenital exocrine pancreatic insufficiency, multiple malformations such as nasal wing aplasia, and frequent mental retardation. Pancreas of individuals with JBS do not express UBR1 and show intrauterine-onset destructive pancreatitis.
  • Sequence similarities
    Belongs to the UBR1 family.
    Contains 1 RING-type zinc finger.
    Contains 1 UBR-type zinc finger.
  • Developmental stage
    Expressed in fetal pancreas.
  • Domain
    The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase UBR1 antibody
    • JBS antibody
    • N-recognin-1 antibody
    • ubiquitin protein ligase E3 component n-recognin 1 antibody
    • Ubiquitin-protein ligase E3-alpha-1 antibody
    • Ubiquitin-protein ligase E3-alpha-I antibody
    • UBR1 antibody
    • UBR1_HUMAN antibody
    see all

Anti-UBR1 antibody images

  • All lanes : Anti-UBR1 antibody (ab42420) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 20 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 200 kDa
    Observed band size : 200 kDa
    Additional bands at : 100 kDa (possible isoform,post-translational modification),93 kDa (possible isoform).

References for Anti-UBR1 antibody (ab42420)

This product has been referenced in:
  • Leng S  et al. Implication of a Chromosome 15q15.2 Locus in Regulating UBR1 and Predisposing Smokers to MGMT Methylation in Lung. Cancer Res 75:3108-17 (2015). Read more (PubMed: 26183928) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab42420.
Please use the links above to contact us or submit feedback about this product.


Sign up