Overview

  • Product name
  • Description
    Rabbit polyclonal to UBR1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human UBR1.

    (Peptide available as ab42419.)

  • Positive control
    • Skeletal Muscle (Human) Tissue Lysate - adult normal tissue HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa).

Target

  • Function
    E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth.
  • Tissue specificity
    Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Defects in UBR1 are a cause of Johanson-Blizzard syndrome (JBS) [MIM:243800]. This disorder includes congenital exocrine pancreatic insufficiency, multiple malformations such as nasal wing aplasia, and frequent mental retardation. Pancreas of individuals with JBS do not express UBR1 and show intrauterine-onset destructive pancreatitis.
  • Sequence similarities
    Belongs to the UBR1 family.
    Contains 1 RING-type zinc finger.
    Contains 1 UBR-type zinc finger.
  • Developmental stage
    Expressed in fetal pancreas.
  • Domain
    The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase UBR1 antibody
    • JBS antibody
    • N-recognin-1 antibody
    • ubiquitin protein ligase E3 component n-recognin 1 antibody
    • Ubiquitin-protein ligase E3-alpha-1 antibody
    • Ubiquitin-protein ligase E3-alpha-I antibody
    • UBR1 antibody
    • UBR1_HUMAN antibody
    see all

Images

  • All lanes : Anti-UBR1 antibody (ab42420) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 200 kDa
    Observed band size : 200 kDa
    Additional bands at : 100 kDa (possible isoform,post-translational modification),93 kDa (possible isoform).

References

This product has been referenced in:
  • Leng S  et al. Implication of a Chromosome 15q15.2 Locus in Regulating UBR1 and Predisposing Smokers to MGMT Methylation in Lung. Cancer Res 75:3108-17 (2015). Read more (PubMed: 26183928) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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