The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
RelevanceUBR2 (Ubiquitin protein ligase E3 component n-recognin 2) is an E3 ubiquitin-protein ligase involved in the N-end rule pathway. It recognizes and binds to proteins with destabilizing N-terminal residues (an N-terminal degron), leading to their ubiquitination and subsequent degradation.