Overview

  • Product name
  • Description
    Rabbit polyclonal to UBR4
  • Tested applications
    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Dog, Turkey, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Opossum, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 150-200 of Human UBR4 (NP_065816.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab114109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the UBR4 family.
      Contains 1 UBR-type zinc finger.
    • Cellular localization
      Membrane. Cytoplasm. Cytoplasm > cytoskeleton. Nucleus. Concentrates at the leading edge of membrane structures involved in actin motility.
    • Information by UniProt
    • Database links
    • Alternative names
      • 600 kDa retinoblastoma protein associated factor antibody
      • 600 kDa retinoblastoma protein-associated factor antibody
      • E3 ubiquitin-protein ligase UBR4 antibody
      • KIAA0462 antibody
      • KIAA1307 antibody
      • N-recognin-4 antibody
      • p600 antibody
      • RBAF600 antibody
      • Retinoblastoma associated factor 600 like protein antibody
      • Retinoblastoma associated factor of 600 kDa antibody
      • Retinoblastoma-associated factor 600 antibody
      • Retinoblastoma-associated factor of 600 kDa antibody
      • Ubiquitin protein ligase E3 component n recognin 4 antibody
      • UBR4 antibody
      • UBR4_HUMAN antibody
      • Zinc finger UBR1 type protein 1 antibody
      • Zinc finger UBR1-type protein 1 antibody
      • Zinc finger, UBR1 type 1 antibody
      • ZUBR 1 antibody
      • ZUBR1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UBR4 with ab114109 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Human UBR4 by Western blot of Immunoprecipitate.
      UBR4 was immunoprecipitated from HeLa whole cell lysate, using ab114109 at 10 µg/mg lysate (1 mg for IP, 20% of IP loaded). An affinity purified rabbit anti-UBR4 antibody was used for the subsequent WB at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab114109 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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