The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 4 - 8 µg/ml.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceUBXD2 is an integral membrane protein of the endoplasmic reticulum (ER) that binds valosin-containing protein (VCP) and promotes ER-associated protein degradation (ERAD).
Immunohistochemical staining of brain tissue from patients with Alzheimer's disease and control subjects revealed that UBXD2 accumulates preferentially in neurons undergoing neurofibrillary degeneration in Alzheimer's disease.
Cellular localizationEndoplasmic reticulum membrane. Both the N- and the C-terminus face the cytosol; also found in the nucleus envelope contiguous to the ER.