Overview

  • Product name
    Anti-UBXN6 antibody [5C3-1]
    See all UBXN6 primary antibodies
  • Description
    Mouse monoclonal [5C3-1] to UBXN6
  • Specificity
    ab80659 recognizes both isoforms of UBXN6
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant Human UBXN6 purified from bacteria.

  • Positive control
    • U2OS cotransfected with pBabepuro and pSuper based plasmid encoding anti UBXD1 shRNA

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, 0.5% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    5C3-1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 0.05 - 0.5 µg/ml.
    IP: Use at 1-2µg/500 3µg protein extract.
    WB: Use at a concentration of 0.3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Acts in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation.
    • Tissue specificity
      Enhanced expression in testis.
    • Sequence similarities
      Contains 1 PUB (PUG) domain.
      Contains 1 UBX domain.
    • Domain
      The UBX domain lacks key residues critical for VCP binding.
    • Cellular localization
      Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes at the centrosome both in interphase and during mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • UBX domain containing 1 antibody
      • UBX domain containing 2 antibody
      • UBX domain containing protein 1 antibody
      • UBX domain containing protein 6 antibody
      • UBX domain protein 6 antibody
      • UBX domain-containing protein 1 antibody
      • UBX domain-containing protein 6 antibody
      • UBXD1 antibody
      • UBXDC2 antibody
      • Ubxn6 antibody
      • UBXN6_HUMAN antibody
      see all

    Images

    • Lanes 1 - 2 : Anti-UBXN6 antibody [5C3-1] (ab80659) at 0.3 µg/ml (in PBS/Tween)
      Lanes 3 - 4 : Ponceau S Red

      Lane 1 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNA
      Lane 2 : U2OS were co-transfected with mismatch control
      Lane 3 : U2OS were co-transfected with pBabepuro and pSuper based plasmid encoding anti- UBXD1 shRNA
      Lane 4 : U2OS were co-transfected with mismatch control


      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 49 kDa (possible isoform).A picture of the Ponceau S stained blot after processing is shown to demonstrate equivalent loading of protein.

    References

    ab80659 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab80659.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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