Overview

  • Product nameAnti-UBXN6 antibody
    See all UBXN6 primary antibodies
  • Description
    Rabbit polyclonal to UBXN6
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residue 391 and 441 of Human UBXN6 (NP_079517.1).

  • Positive control
    • HeLa whole cell lysate 293T whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab103526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActs in a complex with VCP and cooperates with USP7 in promoting MDM2 deubiquitination and stabilization. MDM2 stabilization leads to MDM2-dependent TP53 degradation.
  • Tissue specificityEnhanced expression in testis.
  • Sequence similaritiesContains 1 PUB (PUG) domain.
    Contains 1 UBX domain.
  • DomainThe UBX domain lacks key residues critical for VCP binding.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes at the centrosome both in interphase and during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • UBX domain containing 1 antibody
    • UBX domain containing 2 antibody
    • UBX domain containing protein 1 antibody
    • UBX domain containing protein 6 antibody
    • UBX domain protein 6 antibody
    • UBX domain-containing protein 1 antibody
    • UBX domain-containing protein 6 antibody
    • UBXD1 antibody
    • UBXDC2 antibody
    • Ubxn6 antibody
    • UBXN6_HUMAN antibody
    see all

Anti-UBXN6 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling UBXN6 with ab103526 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-UBXN6 antibody (ab103526) at 0.1 µg/ml

    Lane 1 : HeLa Whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 50 kDa


    Exposure time : 3 minutes
  • Detection of UBXN6 by Western Blot of Immunprecipitate.
    Lane 1: ab103526 at 1µg/ml staining UBXN6 in HeLa whole cell lysate immunoprecipitated using ab103526 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-UBXN6 antibody (ab103526)

ab103526 has not yet been referenced specifically in any publications.

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