Overview

  • Product nameAnti-UCH37 antibody [EPR4896]
    See all UCH37 primary antibodies
  • Description
    Rabbit monoclonal [EPR4896] to UCH37
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in Human UCH37.

  • Positive control
    • Human fetal kidney, 293T, NCCIT and SH-SY5Y cell lysates; Human kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 38 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Antigen retrieval is recommended. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC.
  • Target

    • FunctionProtease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.
    • Sequence similaritiesBelongs to the peptidase C12 family.
    • Cellular localizationCytoplasm. Nucleus. Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • UCH37 antibody
      • AD-019 antibody
      • CGI 70 antibody
      • INO80 complex subunit R antibody
      • INO80R antibody
      • Ubiquitin C terminal hydrolase UCH37 antibody
      • Ubiquitin C-terminal hydrolase UCH37 antibody
      • Ubiquitin carboxyl terminal esterase L5 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L5 antibody
      • Ubiquitin carboxyl terminal hydrolase L5 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L5 antibody
      • Ubiquitin thioesterase L5 antibody
      • Ubiquitin thiolesterase L5 antibody
      • UCH L5 antibody
      • UCH-L5 antibody
      • UCHL5 antibody
      • UCHL5_HUMAN antibody
      see all

    Anti-UCH37 antibody [EPR4896] images

    • All lanes : Anti-UCH37 antibody [EPR4896] (ab124931) at 1/1000 dilution

      Lane 1 : Human fetal kidney cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : NCCIT cell lysates
      Lane 4 : SH-SY5Y cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 38 kDa
    • ab124931, at 1/500 dilution, staining UCH37 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    References for Anti-UCH37 antibody [EPR4896] (ab124931)

    ab124931 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124931.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"