Overview

  • Product nameAnti-UCHL1+3 antibody
  • Description
    Rabbit polyclonal to UCHL1+3
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human protein purified from E.coli (Tagged-UCHL1)

  • Positive control
    • SK-N-MC cell, IMR-32 cell, Mouse brain and Rat brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab75275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/2000. Predicted molecular weight: 25 kDa.

Target

  • RelevanceThe two ubiquitin C-terminal hydrolase (UCH) enzymes, UCHL1 and UCHL3, deubiquitinate ubiquitin-protein conjugates and control the cellular balance of ubiquitin. UCHL1 and UCHL3 are both small proteins of ~220 amino acids that share more than 40% amino acid sequence identity. UCHL3 is universally expressed in all tissues, while UCHL1 is expressed exclusively in neuronal tissue, testis and ovary. The activity of UCHL3 is more than 200 fold higher than UCHL1 when a fluorogenic ubiquitin substrate is used. UCHL1 associates with monoubiquitin and UCHL3 binds to Nedd8, ubiquitin-like protein. UCHL1 and UCHL3 play a role in the regulation of neuronal development and spermatogenesis. UCHL1 is involved in the pathogenesis of Parkinson’s disease (PD) and Alzheimer’s disease (AD).
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal esterase L3 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L3 antibody
    • Ubiquitin thiolesterase antibody
    • UCHL1 antibody
    • UCHL3 antibody
    see all

Anti-UCHL1+3 antibody images

  • All lanes : Anti-UCHL1+3 antibody (ab75275) at 1/2000 dilution

    Lane 1 : SK-N-MC cell lysate
    Lane 2 : IMR-32 cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate


    Predicted band size : 25 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

References for Anti-UCHL1+3 antibody (ab75275)

ab75275 has not yet been referenced specifically in any publications.

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