Overview

  • Product nameAnti-UCHL3 antibody
    See all UCHL3 primary antibodies
  • Description
    Rabbit polyclonal to UCHL3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (EGQRWLPLEA NPEVTNQFLK QLGLHPNWQF VDVYGMDPEL LSMVPRPVCA) of Human UCHL3 (NP_005993).

  • Positive control
    • MCF7 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab87154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionDeubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
  • Tissue specificityHighly expressed in heart, skeletal muscle, and testis.
  • Sequence similaritiesBelongs to the peptidase C12 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
    • Ubiquitin thioesterase L3 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L3 antibody
    • UCH L3 antibody
    • UCH-L3 antibody
    • UCHL3 antibody
    • UCHL3_HUMAN antibody
    see all

Anti-UCHL3 antibody images

  • Anti-UCHL3 antibody (ab87154) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • ab87154 at 5 µg/ml staining UCHL3 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-UCHL3 antibody (ab87154)

ab87154 has not yet been referenced specifically in any publications.

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