- Datasheet
- Specific References
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Overview
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Product nameAnti-UCHL3 antibody
See all UCHL3 primary antibodies -
DescriptionRabbit polyclonal to UCHL3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
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Immunogen
Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (EGQRWLPLEA NPEVTNQFLK QLGLHPNWQF VDVYGMDPEL LSMVPRPVCA) of Human UCHL3 (NP_005993).
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Positive control
- MCF7 cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab87154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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FunctionDeubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
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Tissue specificityHighly expressed in heart, skeletal muscle, and testis.
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Sequence similaritiesBelongs to the peptidase C12 family.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 395626 Chicken
- Entrez Gene: 520170 Cow
- Entrez Gene: 7347 Human
- Entrez Gene: 50933 Mouse
- Entrez Gene: 768115 Pig
- Entrez Gene: 498560 Rat
- Entrez Gene: 554104 Zebrafish
- Omim: 603090 Human
see all -
Alternative names
- Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
- Ubiquitin thioesterase L3 antibody
- Ubiquitin thiolesterase antibody
see all
Images
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Western blot - UCHL3 antibody (ab87154)Anti-UCHL3 antibody (ab87154) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
12% gel -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody (ab87154)ab87154 at 5 µg/ml staining UCHL3 in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References
ab87154 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"