Anti-UCHL3 antibody (ab87154)

Overview

• Product name
  Anti-UCHL3 antibody
  See all UCHL3 primary antibodies
• Description
  Rabbit polyclonal to UCHL3
• Tested applications
  Suitable for: WB, IHC-Pmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
• Immunogen

  Synthetic peptide corresponding to a region within the N terminal amino acids 2-51 (EGQRWLPLEA NPEVTNQFLK QLGLHPNWQF VDVYGMDPEL LSMVPRPVCA) of Human UCHL3 (NP_005993).

• Positive control
  • MCF7 cell lysate.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage buffer
  Preservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Deubiquitination

  • Epigenetics and Nuclear Signaling
  • Ubiquitin & Ubiquitin Like Modifiers
  • Deubiquitination

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory.
• Tissue specificity
  Highly expressed in heart, skeletal muscle, and testis.
• Sequence similarities
  Belongs to the peptidase C12 family.
• Post-translational
  modifications
  Phosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localization
  Cytoplasm.

• Target information above from: UniProt accession P15374 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 395626 Chicken
  • Entrez Gene: 520170 Cow
  • Entrez Gene: 7347 Human
  • Entrez Gene: 50933 Mouse
  • Entrez Gene: 768115 Pig
  • Entrez Gene: 498560 Rat
  • Entrez Gene: 554104 Zebrafish
  • Omim: 603090 Human

  • SwissProt: Q2TBG8 Cow
  • SwissProt: P15374 Human
  • SwissProt: Q9JKB1 Mouse
  • SwissProt: Q91Y78 Rat
  • Unigene: 162241 Human
  • Unigene: 275970 Mouse
  • Unigene: 145487 Rat

  see all
• Alternative names
  • Ubiquitin carboxyl-terminal hydrolase isozyme L3 antibody
  • Ubiquitin thioesterase L3 antibody
  • Ubiquitin thiolesterase antibody

  • Ubiquitin thiolesterase L3 antibody
  • UCH L3 antibody
  • UCH-L3 antibody
  • UCHL3 antibody
  • UCHL3_HUMAN antibody

  see all

Protocols

• Western blot protocols
• Immunohistochemistry protocols