The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
UCK2 phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This is the first step in the production of the pyrimidine nucleoside triphosphates required for RNA and DNA synthesis. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides.