Overview

  • Product nameAnti-UCKL1 antibody
    See all UCKL1 primary antibodies
  • Description
    Rabbit polyclonal to UCKL1
  • Tested applicationsSuitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Chimpanzee, Gorilla, Opossum, Chinese Hamster, Orangutan, Bat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 50-100 of Human UCKL1 (NP_060329.2)

  • Positive control
    • HeLa whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab95177 was affinity purified using an epitope specific to UCKL1 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionMay contribute to UTP accumulation needed for blast transformation and proliferation.
    • Tissue specificityUbiquitous.
    • PathwayPyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.
      Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
    • Sequence similaritiesBelongs to the uridine kinase family.
    • Post-translational
      modifications
      Ubiquitinated by RNF19B; which induces proteasomal degradation.
    • Cellular localizationCytoplasm. Nucleus. EBNA3 induces isoform 1 translocation to the nucleus, whereas it does change isoform 3 location.
    • Information by UniProt
    • Database links
    • Alternative names
      • F538 antibody
      • UCK 1L antibody
      • UCK1 like antibody
      • UCK1L antibody
      • UCKL 1 antibody
      • UCKL1 antibody
      • UCKL1_HUMAN antibody
      • Uridine cytidine kinase 1 like 1 antibody
      • Uridine kinase like 1 antibody
      • Uridine-cytidine kinase-like 1 antibody
      • Uridine/cytidine kinase like 1 antibody
      • URKL 1 antibody
      • URKL1 antibody
      see all

    Anti-UCKL1 antibody images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling UCKL1 with ab95177 at 1/1000 (1µg/mg). Detection: DAB.
    • Immunoprecipitation of UCKL1 from HeLa whole cell lysate (1 mg) using ab95177 at 3µg/mg lysate followed by Western blotting of 20% of the immunoprecipitate: (1) ab95177 at 1µg/ml, (2) control IgG
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-UCKL1 antibody (ab95177)

    ab95177 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab95177.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"