Overview

  • Product name
  • Description
    Rabbit polyclonal to UCP1
  • Specificity
    ab10983 has been found not to work in human samples in western blotting, but was shown to work in IHC-P and ICC/IF. The antibody does not cross-react with UCP2 or UCP3.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Spermophilus tridecemlineatus
    Predicted to work with: Sheep, Rabbit, Cow, Dog, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 145-159 of Human UCP1, with N-terminal cysteine added.

  • Positive control
    • Brown adipose tissue.
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab10983 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500. PubMed: 22545021
WB 1/1000. Detects a band of approximately 32 kDa. Use at a minimum dilution of 1/1000 in extract of rat brown adipose tissue (BAT) mitochondria or an extract of E.coli expressing recombinant mouse UCP1. Additional weak bands may be detected in some preparations of BAT extracts. Staining of the UCP1 band is specifically inhibited with the immunizing peptide. This antibody may not be suitable for Western blot in Human samples.
IHC-P 1/500. This concentration is determined by indirect immunoperoxidase staining of protease digested, formalin-fixed, paraffin-embedded sections of mouse brown adipose tissue.

Target

  • Function
    UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificity
    Brown adipose tissue.
  • Sequence similarities
    Belongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Form
    UCP1 is preferentially expressed in brown adipose tissue
  • Alternative names
    • mitochondrial brown fat uncoupling protein antibody
    • Mitochondrial brown fat uncoupling protein 1 antibody
    • SLC25A7 antibody
    • Solute carrier family 25 member 7 antibody
    • Thermogenin antibody
    • UCP 1 antibody
    • UCP antibody
    • UCP1 antibody
    • UCP1_HUMAN antibody
    • uncoupling protein 1 (mitochondrial, proton carrier) antibody
    • Uncoupling protein 1 antibody
    see all

Anti-UCP1 antibody images

  • All lanes : Anti-UCP1 antibody (ab10983) at 1 µg/ml

    Lane 1 : Rat brown fat
    Lane 2 : Rat brown fat with UCP1 immunizing peptide (human, 145-159)

    Secondary
    Goat Anti-Rabbit IgG-Alkaline Phosphatase and a colorimetric substrate
  • ab10983 staining UCP1 in Human brown adipose tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% triton for 20 minutes and blocked with 10% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer, pH 6. Samples were incubated with primary antibody (1/250 in PBS + 1% BSA) for 18 hours at 4°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab10983, staining UCP1 (green), in undifferentiated (left) and differentiated (right) murine fat progenitor cells, by Immunocytochemistry/ Immunofluorescence.

    Cells were incubated with primary antibody at 1/500 dilution. An AlexaFluor®546-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained using DAPI (blue).
  • ab10983, staining UCP1, in murine inguinal white adipose tissue, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was incubated with primary antibody at 1/1000 dilution and staining was detected using an HRP/DAB detection kit. Sections were counterstained using hematoxylin.

References for Anti-UCP1 antibody (ab10983)

This product has been referenced in:
  • Wu CS  et al. Suppression of GHS-R in AgRP Neurons Mitigates Diet-Induced Obesity by Activating Thermogenesis. Int J Mol Sci 18:N/A (2017). WB ; Mouse . Read more (PubMed: 28420089) »
  • Yuan JH  et al. Nesfatin-1 in the Lateral Parabrachial Nucleus Inhibits Food Intake, Modulates Excitability of Glucosensing Neurons, and Enhances UCP1 Expression in Brown Adipose Tissue. Front Physiol 8:235 (2017). WB ; Rat . Read more (PubMed: 28484396) »

See all 96 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DU145 Prostate cancer cell line)
Permeabilization
Yes - 0.1% v/v TritonX-100
Specification
DU145 Prostate cancer cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Feb 06 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (mesenchymal stem cells)
Permeabilization
Yes - Triton 0.25%
Specification
mesenchymal stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 04 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (fetal visceral fat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
fetal visceral fat
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Username

Mr. Jeremy Gingrich

Verified customer

Submitted Apr 15 2016

Application
Western blot
Sample
Sheep Cell lysate - whole cell (preadipocyte)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% PAG)
Loading amount
10 µg
Specification
preadipocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Jeremy Gingrich

Verified customer

Submitted Apr 15 2016

Application
Western blot
Sample
Sheep Cell lysate - whole cell (Ovine brown adipocyte precursor cells)
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Loading amount
30 µg
Treatment
9 day induction of differentiation with a serum-free Dulbecco's modified Eagle Ham/F12 medium supplemented with 100 nM dexamethasone, 1 μg/mL insulin, 10 pg/mL transferrin, and 1 nM triiodothyronine
Specification
Ovine brown adipocyte precursor cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brown adipose tissue)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Brown adipose tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 27 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization
No
Specification
breast tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% buffered formalin
Username

Abcam user community

Verified customer

Submitted Aug 17 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate 95C
Permeabilization
Yes - PBS with 3% Triton
Specification
Mammary gland
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 04 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brown fat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6
Permeabilization
No
Specification
Brown fat
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin
Username

Abcam user community

Verified customer

Submitted Jul 20 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (White and brown fat)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6 citrate
Permeabilization
No
Specification
White and brown fat
Blocking step
Dako dual enzyme and protein blocks as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
Formaldehyde
Username

Dr. Jean-Martin Lapointe

Verified customer

Submitted Jul 13 2015

1-10 of 27 Abreviews or Q&A

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