Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human UCP1.
(Peptide available as ab24282.)
Our Abpromise guarantee covers the use of ab23841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||Use at an assay dependent concentration.|
ab23841 staining UCP1 in serial sections of white fat from mice by immunohistochemistry. Serial sections were de-paraffinized, rehydrated through xylene, ethanol and water; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in blocking buffer) for 60 minutes. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody.