Overview

  • Product nameAnti-UCP2 antibody
    See all UCP2 primary antibodies
  • Description
    Mouse polyclonal to UCP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-310 of Human UCP2

  • Positive control
    • Human ovary and Rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

Target

  • FunctionUCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
  • Tissue specificityWidely expressed in adult human tissues, including tissues rich in macrophages. Most expressed in white adipose tissue and skeletal muscle.
  • Sequence similaritiesBelongs to the mitochondrial carrier family.
    Contains 3 Solcar repeats.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMIQ4 antibody
    • Mitochondrial uncoupling protein 2 antibody
    • SLC25A8 antibody
    • Solute carrier family 25 member 8 antibody
    • UCP 2 antibody
    • ucp2 antibody
    • UCP2_HUMAN antibody
    • UCPH antibody
    • Uncoupling protein 2 antibody
    • Uncoupling protein 2 mitochondrial proton carrier antibody
    see all

Anti-UCP2 antibody images

  • All lanes : Anti-UCP2 antibody (ab67241) at 1/500 dilution

    Lane 1 : UCP2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
  • Anti-UCP2 antibody (ab67241) at 1/500 dilution + rat brain tissue lysate at 25 µg/ml

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa

References for Anti-UCP2 antibody (ab67241)

This product has been referenced in:
  • Yuan Y  et al. MiR-133a Is Functionally Involved in Doxorubicin-Resistance in Breast Cancer Cells MCF-7 via Its Regulation of the Expression of Uncoupling Protein 2. PLoS One 10:e0129843 (2015). WB ; Human . Read more (PubMed: 26107945) »
  • Nie D  et al. The Stress-Induced Atf3-Gelsolin Cascade Underlies Dendritic Spine Deficits in Neuronal Models of Tuberous Sclerosis Complex. J Neurosci 35:10762-72 (2015). Mouse . Read more (PubMed: 26224859) »

See all 7 Publications for this product

Product Wall

Thank you for your reply.

Unfortunately due to Canadian custom restrictions we are unable to send you ab77363. I do apologize for this inconvenience but please let me know if there is another antibody that you would like to receive as a free ...

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Thank you for your reply.

Regarding the UCP3 results, I agree that the lower band would be the UCP# signal but I am not sure what the higher molecular weight band could be. It may just be non-specific binding of the antibody, or it may be pos...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 20 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Sep 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT-29,SW480,Hep2,Hela,HCT116)
Loading amount 30 µg
Specification HT-29,SW480,Hep2,Hela,HCT116
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. YAN NAN SHEN

Verified customer

Submitted Jun 28 2012

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided...

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Thank you for your inquiry.
Unfortunately, this antibody was not tested for its cross-reactivity to UCP1 and UCP2 experimentally.
We usually assume a cross-reaction when the homology is above 85%. As ab67241 a polyclonal antibody and the full...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"